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KMID : 0365819640040010001
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Journal of Pusan Medical College
1964 Volume.4 No. 1 p.1 ~ p.14
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Experimental Study of Procaine Therapy
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Abstract
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Reports on the effect of procaine hydrochloride in various inflammatory diseases was first described by A. B. Bishnevsky in 1929 who stated that the inflammation of the gall bladder subsided with the local infiltration of procaine hydrochloride alone, after he failed to perform cholecystectomy on acute cholecystitis under local anesthesia with procaine hydrochloride.
The use of procaine hydrochloride for the treatment of inflammatory diseases has been widely practiced in our clinic. Especially in the treatment of acute cholecystitis, the procaine therapy revealed marked usefulness in deciding the surgical indication and the most suitable time for the operation. It also improves the post operative course and sometimes it retakes surgery unnecessary.
Since histologic study of cholecystitis in human cases revealed evidence of allergic nature the writer made an experimental study on the effect of procaine therapy upon experimentally induced allergic cholecystitis in 60 rabbits.
Experimental methods;
Healthy rabbits weighing about 2 Kg were used. Fresh´ normal horse serum was used as the antigen to induce allergic cholecystitis.
the horse serum (1 cc/kg of body wt.) was injected subcutane-jusly into the abdominal wall on four consecutive days. On the 15th day, after the last sensitization, 0. 1cc per k, of body weight of the serum was injected into the perimuscular layer of the gall bladder wall.
The animals were divided into 6 groups.
1st group; Unsensitized for the control rabbits.
2nd group; Reinjected after sensitization, and Ringer´s solution injected for the sake of control to procaine therapy.
3rd group; Reinjected after sensitization, and treated with procaine therapy. 4th group; Cholecystic duct ligated for the control.
5th group; Reinjected after sensitization, cholecystic duct ligated, and Ringer´s solution
injected for the control to procaine therapy.
6th group; Reinjected after sensitization, cholecystic duct ligated, and treated with
procaine therapy.
After performing cholecystectomy on every case, patho-histological study of the gall bladder were made.
Results;
The histological effect of procaine hydrochloride: upon experimental allergy were found as follows:
1. The gall bladder of allergic state was found to have remarkable vascular reactions of congestion, hemorrhage, edema, and infiltration of manocytes and eosinophils. It also showed moderate hyperplasia of mucosae epithelium and minor necrosis (2n1 group).
2. In more severe allergic cases; induced by ligation of cholecystic duct, the changes were moderate vascular reactions and marked necrosis (5th group).
3. Histological changes of allergic cholecystitis treated with procaine hydrochloride showed minimal vascular reactions and an abscence of necrosis in moderate cases (3rd group).
4. For the severe cases of allergic cholecystitis with ligation of cholecystic duct, the
procaine therapy caused decrease of necrosis and vascular reactions (6th group).
5. In summary, procaine hydrochloride inhibited and caused cessation of allergic inflammatory reactions of the gall bladder in the patho-histological processes.
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